Reverse Transcription-based Loop Mediated Isothermal Amplification (RT-LAMP)

•It is a simple quantitative detection method.
•In this method, a DNA copy of the viral RNA is generated by reverse transcriptase, and then isothermal amplification is carried out to increase the amount of total DNA.
•A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (DNA) from an RNA template, a process termed reverse transcription.
•Isothermal amplification enables rapid and specific amplification of DNA at constant temperature (60-65 °C).